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Journal: Translational Oncology
Article Title: Circular RNA circSCMH1 regulates glycolysis to inhibit gastric cancer metastasis via miR-296–3p/HSPB7-GLUT3 axis
doi: 10.1016/j.tranon.2025.102644
Figure Lengend Snippet: CircSCMH1 sponged miR-296–3p which facilitate migration and proliferation in GC cells. (A) Heatmap of miRNAs showed opposite alternation with circSCMH1 in GC tissue (High expression: circSCMH1 high-expressed samples; Low expression: circSCMH1 low-expressed samples; N: normal tissue, T: tumor tissue). (B) Relative expression of three potential target miRNAs in circSCMH1 knockdown GC cells. (C) Dual-luciferase report assays in GC cells showed circSCMH1 sponging with mi-296–3p (D) RIP assay performed with anti-AGO2 antibody to evaluate the combination of circSCMH1 and miR-296–3p (E, F) Trans-well assay and CCk8 assay showed miR-296–3p accelerate migration and proliferation of GC cells. All data was shown as mean ± SD value. * P < 0.05, ** P < 0.01, *** P < 0.001; ns stands for no significance.
Article Snippet:
Techniques: Migration, Expressing, Knockdown, Luciferase, CCK-8 Assay